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Clinical characteristics and prognostic values of 1p32.3 deletion detected through fluorescence

Huanping Wang, Haitao Meng, Jinghan Wang, Yinjun Lou, Yile Zhou, Peipei Lin, Fenglin Li, Lin Liu, Huan Xu, Min Yang, Jie Jin

《医学前沿（英文）》 2020年 第14卷 第3期   页码 327-334 doi: 10.1007/s11684-019-0712-x

摘要： This study aimed to investigate the prevalence, clinical characteristics, and prognostic impact of 1p32.3 deletion in patients with newly diagnosed multiple myeloma (MM). A retrospective analysis was conducted on 411 patients with newly diagnosed MM; among which, 270 received bortezomib-based therapies, and 141 received thalidomide-based therapies. Fluorescence hybridization (FISH) was performed to detect six cytogenetic abnormalities, namely, del(1p32.3), gain(1q21), del(17p13), del(13q14), t(4;14), and t(11;14). Results showed that 8.3% of patients with MM were detected with del(1p32.3) and had significantly more bone marrow plasma cells ( = 0.025), higher 2-microglobulin levels ( = 0.036), and higher lactate dehydrogenase levels ( = 0.042) than those without del(1p32.3). Univariate analysis showed that patients with del(1p32.3) under thalidomide-based therapies (median PFS 11.6 vs. 31.2 months, = 0.002; median OS 16.8 vs. 45.9 months, <0.001) were strongly associated with short progression-free survival (PFS) ( = 0.002) and overall survival (OS) ( <0.001). Multivariate analysis revealed that del(1p32.3) remained a powerful independent factor with worse PFS ( = 0.006) and OS ( = 0.016) for patients under thalidomide-based treatments. Patients with del(1p32.3) under bortezomib-based treatments tended to have short PFS and OS. In conclusion, del(1p32.3) is associated with short PFS and OS in patients with MM who received thalidomide- or bortezomib-based treatments.

关键词： 1p32.3 deletion     1q21 gain     prognosis     multiple myeloma     FISH     bortezomib     thalidomide    

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The predictive value of chromosome 8p deletion for metastasis of hepatocellular carcinoma: a summary

QIN Lunxiu, TANG Zhaoyou, GUAN Xinyuan, YE Qinghai, JIA Huliang, REN Ning

《医学前沿（英文）》 2008年 第2卷 第3期   页码 211-215 doi: 10.1007/s11684-008-0041-y

摘要： Hepatocellular carcinoma (HCC) represents an extremely poor prognostic cancer, which is mainly due to the high frequency of metastasis/recurrence after surgical operation. Exploring the molecular mechanisms involved in HCC metastasis could be helpful in the prediction and early diagnosis of HCC recurrence and could also provide new therapeutic targets for HCC metastasis. In the recent decade, we analyzed the genomic aberrations of the clinical specimens, as well as the metastatic models and cell lines of human HCC to identify the genetic markers related to HCC metastasis and to verify their clinical values in the prediction and control of metastasis of HCC. Using the comparative genomic hybridization (CGH) technique, we compared the differences of chromosomal aberrations between primary HCC tumors and their matched metastatic lesions, and found that chromosome 8p deletions might contribute to HCC metastasis. This novel finding was further confirmed by comparison between nude mice models of HCC with different metastatic potentials. By the more sensitive genome-wide microsatellite analysis, 8p deletion was defined to 8p23.3 and 8p11.2, which are two likely regions harboring metastasis-related genes of HCC. Using ‘8p-specific’ microarrays, two novel metastatic suppressors ( and ) were identified, and were proven to suppress invasion and metastasis of HCC. Clinical studies indicate that 8p deletion detected in HCC or circulating plasma DNA of patients is a useful predictor for metastatic recurrence and prognosis, even for patients with early stage HCC. These novel findings are regarded as important advances in the study of the molecular mechanisms of HCC metastasis, which provide not only a holistic view on the molecular cytogenetic bases of HCC metastasis, but also candidate regions for further study to identify metastatic suppressor genes.

关键词： sensitive genome-wide     prediction     genome-wide microsatellite     frequency     Hepatocellular carcinoma    

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RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

《农业科学与工程前沿（英文）》 2016年 第3卷 第1期   页码 81-86 doi: 10.15302/J-FASE-2016088

摘要： As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

关键词： pseudorabies virus     RR1 and RR2     ribonucleotide reductase     vaccine candidate    

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Probes and nano-delivery systems targeting NAD(P)H:quinone oxidoreductase 1: a mini-review

《化学科学与工程前沿（英文）》 2023年 第17卷 第2期   页码 123-138 doi: 10.1007/s11705-022-2194-7

摘要： The two-electron cytoplasmic reductase NAD(P)H:quinone oxidoreductase 1 is expressed in many tissues. NAD(P)H:quinone oxidoreductase 1 is well-known for being highly expressed in most cancers. Therefore, it could be a target for cancer therapy. Because it is a quinone reductase, many bioimaging probes based on quinone structures target NAD(P)H:quinone oxidoreductase 1 to diagnose tumours. Its expression is higher in tumours than in normal tissues, and using target drugs such as β-lapachone to reduce side effects in normal tissues can help. However, the physicochemical properties of β-lapachone limit its application. The problem can be solved by using nanosystems to deliver β-lapachone. This mini-review summarizes quinone-based fluorescent, near-infrared and two-photon fluorescent probes, as well as nanosystems for delivering the NAD(P)H:quinone oxidoreductase 1-activating drug β-lapachone. This review provides valuable information for the future development of probes and nano-delivery systems that target NAD(P)H:quinone oxidoreductase 1.

关键词： NAD(P)H:quinone oxidoreductase 1     cancer therapy     target     probe     nanosystem    

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Keratin 5-Cre-driven deletion of

Jun Yang, Lianqing Wang, Yingzhi Huang, Keqiang Liu, Chaoxia Lu, Nuo Si, Rongrong Wang, Yaping Liu, Xue Zhang

《医学前沿（英文）》 2020年 第14卷 第3期   页码 305-317 doi: 10.1007/s11684-019-0722-8

摘要： Familial acne inversa (AI) is an autoinflammatory disorder that affects hair follicles and is caused by loss-of-function mutations in -secretase component genes. We and other researchers showed that ( ) is the most frequently mutated gene in familial AI. In this study, we generated a keratin 5-Cre-driven epidermis-specific conditional knockout mutant in mice. We determined that this mutant recapitulated the major phenotypes of AI, including hyperkeratosis of hair follicles and inflammation. In mice, the IL-36a expression level markedly increased starting from postnatal day 0 (P0), and this increase occurred much earlier than those of TNF- , IL-23A, IL-1 and TLR4. RNA-Seq analysis indicated that Sprr2d, a member of the small proline-rich protein 2 family, in the skin tissues of the mice was also upregulated on P0. Quantitative reverse-transcription polymerase chain reaction showed that other genes had a similar expression pattern. Our findings suggested that IL-36a might be a key inflammatory cytokine in the pathophysiology of AI and involved in the malfunction of the skin barrier in the pathogenesis of AI.

关键词： acne inversa mouse model     interleukin 1 family     member 6     small proline rich protein 2D     key inflammatory cytokine    

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noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the miR-627-3p/SPRED1 axis

《医学前沿（英文）》   页码 924-938 doi: 10.1007/s11684-023-1004-z

摘要： Long noncoding RNAs (lncRNAs) play a crucial regulatory role in the development and progression of multiple cancers. However, the potential mechanism by which lncRNAs affect the recurrence and metastasis of ovarian cancer remains unclear. In the current study, the lncRNA LOC646029 was markedly downregulated in metastatic ovarian tumors compared with primary tumors. Gain- and loss-of-function assays demonstrated that LOC646029 inhibits the proliferation, invasiveness, and metastasis of ovarian cancer cells in vivo and in vitro. Moreover, the downregulation of LOC646029 in metastatic ovarian tumors was strongly correlated with poor prognosis. Mechanistically, LOC646029 served as a miR-627-3p sponge to promote the expression of Sprouty-related EVH1 domain-containing protein 1, which is necessary for suppressing tumor metastasis and inhibiting KRAS signaling. Collectively, our results demonstrated that LOC646029 is involved in the progression and metastasis of ovarian cancer, which may be a potential prognostic biomarker.

关键词： ovarian cancer     lncRNA LOC646029     metastasis     microRNA 627-3p     SPRED1    

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Synthesis of zeolite Na-P1 from coal fly ash produced by gasification and its application as adsorbent

Yixin Zhang, Lu Zhou, Liqing Chen, Yang Guo, Fanhui Guo, Jianjun Wu, Baiqian Dai

《化学科学与工程前沿（英文）》 2021年 第15卷 第3期   页码 518-527 doi: 10.1007/s11705-020-1926-9

摘要： The coal fly ash produced by gasification is estimated to be over 80 million ton per year in China by 2021. It has mainly been disposed as solid waste by landfill. There is lack of study focused on its utilization. In this paper, the coal fly ash produced by gasification was at first analyzed and then applied to synthesize zeolite as an adsorbent. The effects of synthesis conditions on the cation exchange capacity (CEC) of zeolite were investigated. The results from X-ray diffraction and scanning electron microscope indicated that the crystallinity of the synthesized zeolite is the most important factor to affect the CEC. When the synthesized zeolite with the highest CEC (275.5 meq/100 g) was used for the adsorption of Cr(VI) from aqueous solution, the maximum adsorption capacity for Cr(VI) was found to be 17.924 mg/g. The effects of pH, contact time and initial concentration on the adsorption of Cr(VI) were also investigated. The adsorption kinetics and isotherms can be well described by the pseudo-second-order model and Langmuir isotherm model, respectively.

关键词： coal fly ash     gasification     zeolite     Na-P1     chromium(VI)    

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PAK1 is a novel cardiac protective signaling molecule

null

《医学前沿（英文）》 2014年 第8卷 第4期   页码 399-403 doi: 10.1007/s11684-014-0380-9

摘要：

We review here the novel cardiac protective effects of the multifunctional enzyme, p21-activated kinase 1 (PAK1), a member of a serine/threonine protein kinase family. Despite the large body of evidence from studies in noncardiac tissue indicating that PAK1 activity is key in the regulation of a number of cellular functions, the role of PAK1 in the heart has only been revealed over the past few years. In this review, we assemble an overview of the recent findings on PAK1 signaling in the heart, particularly its cardiac protective effects. We present a model for PAK1 signaling that provides a mechanism for specifically affecting cardiac cellular processes in which regulation of protein phosphorylation states by protein phosphatase 2A (PP2A) predominates. We discuss the anti-adrenergic and antihypertrophic cardiac protective effects of PAK1, as well as its role in maintaining ventricular Ca2+ homeostasis and electrophysiological stability under physiological, β-adrenergic and hypertrophic stress conditions.

关键词： p21-activated kinase 1 (PAK1)     heart    

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用高光谱发光法研究Cu(Inx,Ga1–x)Se2 (CIGS)模块中由P1引起的功率损耗的成因 Article

César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa

《工程（英文）》 2020年 第6卷 第12期   页码 1395-1402 doi: 10.1016/j.eng.2019.12.019

摘要： <p style="text-align: justify;">在本文中，我们利用了高光谱高分辨率光致发光映射技术，这是一个强大的工具，可用于选择和优化在Cu(Inx,Ga1-x)Se2（CIGS）模块上对子电池进行图案化互连的激光烧蚀工艺。具体而言，通过分析在CIGS沉积之前烧蚀的标准P1图案线，我们发现了沿着下部的钼槽边缘的异常发射猝灭效应。通过扫描电子显微镜（SEM）比较了P1边缘的横截面的形貌，我们进一步合理化产生这种效应的起因，但无法用厚度变化解释光发射的减少。我们还研究了激光诱导对CIGS沉积后的P1图案线带来的损伤。我们的发现能更好地理解P1引起的功率损耗，并为改进与行业相关的模块互连方案提供了新的见解。p>

关键词： Cu(Inx     Ga1–x)Se2     电池到模块的效率差距     由P1引起的功率损耗     高光谱光致发光     激光烧蚀短程热效应    

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Adsorption of 1,3-propanediol from synthetic mixture using polymeric resin as adsorbents

TANTAYAKOM, P. PRASITCHOKE, C. MUANGNAPOH

《化学科学与工程前沿（英文）》 2009年 第3卷 第1期   页码 52-57 doi: 10.1007/s11705-009-0087-7

摘要： The aim of this work was to separate 1,3-PDO from a synthetic mixture using polymeric resins, Amberlite XAD-7 and XAD-16 resins. The equilibrium adsorption of 1,3-PDO onto two polymeric resins were investigated in binary and tertiary systems. Experimental results of binary component adsorption equilibrium indicated that the adsorption capacity ( ) of 1,3-PDO at 160 g/L onto XAD-7 and XAD-16 was 835.96 and 584.61 mg 1,3-PDO/g dry resin, respectively. The adsorption isotherms were closely predicted by the Langmuir-Freundlich model among the two isotherm model tested. The value of n of 1,3-PDO adsorbed on XAD-7 are much higher than those on XAD-16. This result suggested that XAD-7 resin has a higher affinity for the 1,3-PDO adsorption than XAD-16 resin. Moreover, the value of adsorption capacity of 1,3-PDO in the binary and tertiary component were compared at the same conditions. In the tertiary system, although the selectivity of 1,3-PDO from XAD-7 was approximately six times higher than XAD-16, the adsorption capacity of 1,3-PDO at 160 g/L onto XAD-16 was higher than XAD-7. Interestingly, the reusability of XAD-7 and XAD-16 resins in the three cycle times shows a slight loss of adsorption capacity. Furthermore, the investigation about desorption by an ethanol/water mixture at 50% ( / ) indicated that the desorption yield of 1,3-PDO from XAD-7 was lower than XAD-16 resin for both the binary and tertiary component. This was due to the more favorable adsorption characteristics of XAD-7 resin than XAD-16 resin.

关键词： adsorption     1     3-propanediol     glycerol     polymeric resin     adsorption isotherm    

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Inhibition of the nuclear export of p65 and IQCG in leukemogenesis by NUP98-IQCG

null

《医学前沿（英文）》 2016年 第10卷 第4期   页码 410-419 doi: 10.1007/s11684-016-0489-0

摘要：

NUP98 fuses with approximately 34 different partner genes via translocation in hematological malignancies. Transgenic or retrovirus-mediated bone marrow transplanted mouse models reveal the leukemogenesis of some NUP98-related fusion genes. We previously reported the fusion protein NUP98-IQ motif containing G (IQCG) in a myeloid/T lymphoid bi-phenoleukemia patient with t(3;11) and confirmed its leukemogenic ability. Herein, we demonstrated the association of NUP98-IQCG with CRM1, and found that NUP98-IQCG expression inhibits the CRM1-mediated nuclear export of p65 and enhances the transcriptional activity of nuclear factor-κB. Moreover, IQCG could be entrapped in the nucleus by NUP98-IQCG, and the fusion protein interacts with calmodulin via the IQ motif in a calcium-independent manner. Therefore, the inhibition of nuclear exports of p65 and IQCG might contribute to the leukemogenesis of NUP98-IQCG.

关键词： NUP98-IQCG     nuclear export     NF-κB     CRM1    

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Decitabine induces -mediated immune responses in p53-mutated triple-negative breast cancer: a clinical

《医学前沿（英文）》 doi: 10.1007/s11684-023-1016-8

摘要： p53 is mutated in half of cancer cases. However, no p53-targeting drugs have been approved. Here, we reposition decitabine for triple-negative breast cancer (TNBC), a subtype with frequent p53 mutations and extremely poor prognosis. In a retrospective study on tissue microarrays with 132 TNBC cases, DNMT1 overexpression was associated with p53 mutations (P = 0.037) and poor overall survival (OS) (P = 0.010). In a prospective DEciTabinE and Carboplatin in TNBC (DETECT) trial (NCT03295552), decitabine with carboplatin produced an objective response rate (ORR) of 42% in 12 patients with stage IV TNBC. Among the 9 trialed patients with available TP53 sequencing results, the 6 patients with p53 mutations had higher ORR (3/6 vs. 0/3) and better OS (16.0 vs. 4.0 months) than the patients with wild-type p53. In a mechanistic study, isogenic TNBC cell lines harboring DETECT-derived p53 mutations exhibited higher DNMT1 expression and decitabine sensitivity than the cell line with wild-type p53. In the DETECT trial, decitabine induced strong immune responses featuring the striking upregulation of the innate immune player IRF7 in the p53-mutated TNBC cell line (upregulation by 16-fold) and the most responsive patient with TNBC. Our integrative studies reveal the potential of repurposing decitabine for the treatment of p53-mutated TNBC and suggest IRF7 as a potential biomarker for decitabine-based treatments.

关键词： p53 mutation     triple-negative breast cancer     decitabine     DNMT1     IRF7     innate immune response    

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Modelling the dynamic response of a solid oxide steam electrolyser to transient inputs during renewable hydrogen production

Qiong CAI, Nigel P. BRANDON, Claire S. ADJIMAN,

《能源前沿（英文）》 2010年 第4卷 第2期   页码 211-222 doi: 10.1007/s11708-010-0037-6

摘要： Hydrogen is regarded as a leading candidate for alternative future fuels. Solid oxide electrolyser cells (SOEC) may provide a cost-effective and green route to hydrogen production especially when coupled to a source of renewable electrical energy. Developing an understanding of the response of the SOEC stack to transient events that may occur during its operation with intermittent electricity input is essential before the realisation of this technology. In this paper, a one-dimensional (1D) dynamic model of a planar SOEC stack has been employed to study the dynamic behaviour of such an SOEC and the prospect for stack temperature control through variation of the air flow rate. Step changes in the average current density from 1.0 to 0.75, 0.5 and 0.2 A/cm have been imposed on the stacks, replicating the situation in which changes in the supply of input electrical energy are experienced, or the sudden switch-off of the stack. Such simulations have been performed both for open-loop and closed-loop cases. The stack temperature and cell voltage are decreased by step changes in the average current density. Without temperature control via variation of the air flow rate, a sudden fall of the temperature and the cell potential occurs during all the step changes in average current density. The temperature excursions between the initial and final steady states are observed to be reduced by the manipulation of the air flow rate. Provided that the change in the average current density does not result in a transition from exothermic to endothermic operation of the SOEC, the use of the air flow rate to maintain a constant steady-state temperature is found to be successful.

关键词： hydrogen production     renewable energy     solid oxide electrolyser cell     1D dynamic model     dynamic behaviour     temperature control    

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A simple umbelliferone based fluorescent probe for the detection of nitroreductase

P. Elmes, Tony D. James

《化学科学与工程前沿（英文）》 2018年 第12卷 第2期   页码 311-314 doi: 10.1007/s11705-017-1697-0

摘要： A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.

关键词： fragmentation     NCOU1     NTR     nitroreductase     nitrobenzyl-umbelliferone    

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标题 作者 时间 类型 操作

Clinical characteristics and prognostic values of 1p32.3 deletion detected through fluorescence

Huanping Wang, Haitao Meng, Jinghan Wang, Yinjun Lou, Yile Zhou, Peipei Lin, Fenglin Li, Lin Liu, Huan Xu, Min Yang, Jie Jin

期刊论文

The predictive value of chromosome 8p deletion for metastasis of hepatocellular carcinoma: a summary

QIN Lunxiu, TANG Zhaoyou, GUAN Xinyuan, YE Qinghai, JIA Huliang, REN Ning

期刊论文

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

期刊论文

Probes and nano-delivery systems targeting NAD(P)H:quinone oxidoreductase 1: a mini-review

期刊论文

Keratin 5-Cre-driven deletion of

Jun Yang, Lianqing Wang, Yingzhi Huang, Keqiang Liu, Chaoxia Lu, Nuo Si, Rongrong Wang, Yaping Liu, Xue Zhang

期刊论文

noncoding RNA LOC646029 functions as a ceRNA to suppress ovarian cancer progression through the miR-627-3p/SPRED1 axis

期刊论文

Synthesis of zeolite Na-P1 from coal fly ash produced by gasification and its application as adsorbent

Yixin Zhang, Lu Zhou, Liqing Chen, Yang Guo, Fanhui Guo, Jianjun Wu, Baiqian Dai

期刊论文

PAK1 is a novel cardiac protective signaling molecule

null

期刊论文

用高光谱发光法研究Cu(Inx,Ga1–x)Se2 (CIGS)模块中由P1引起的功率损耗的成因

César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa

期刊论文

Adsorption of 1,3-propanediol from synthetic mixture using polymeric resin as adsorbents

TANTAYAKOM, P. PRASITCHOKE, C. MUANGNAPOH

期刊论文

Inhibition of the nuclear export of p65 and IQCG in leukemogenesis by NUP98-IQCG

null

期刊论文

Decitabine induces -mediated immune responses in p53-mutated triple-negative breast cancer: a clinical

期刊论文

Modelling the dynamic response of a solid oxide steam electrolyser to transient inputs during renewable hydrogen production

Qiong CAI, Nigel P. BRANDON, Claire S. ADJIMAN,

期刊论文

A simple umbelliferone based fluorescent probe for the detection of nitroreductase

P. Elmes, Tony D. James

期刊论文

construction integration technology in Brazil’s superlarge deep-water offshore oil and gas unit project (FPSO P67/P70)

期刊论文